Evidence for an Upper Limit to Mitotic Spindle Length

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Evidence for an Upper Limit to Mitotic Spindle Length

Size specification of macromolecular assemblies in the cytoplasm is poorly understood [1]. In principle, assemblies could scale with cell size or use intrinsic mechanisms. For the mitotic spindle, scaling with cell size is expected, because the function of this assembly is to physically move sister chromatids into the center of nascent daughter cells. Eggs of Xenopus laevis are among the larges...

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Accurate control of spindle length is a conserved feature of eukaryotic cell division. Lengthening of mitotic spindles contributes to chromosome segregation and cytokinesis during mitosis in animals and fungi. In contrast, spindle shortening may contribute to conservation of egg cytoplasm during female meiosis. Katanin is a microtubule-severing enzyme that is concentrated at mitotic and meiotic...

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Force and Length in the Mitotic Spindle

The mitotic spindle assembles to a steady-state length at metaphase through the integrated action of molecular mechanisms that generate and respond to mechanical forces. While molecular mechanisms that produce force have been described, our understanding of how they integrate with each other, and with the assembly/disassembly mechanisms that regulate length, is poor. We review current understan...

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An allometric relationship between mitotic spindle width, spindle length, and ploidy in Caenorhabditis elegans embryos

The mitotic spindle is a diamond-shaped molecular apparatus crucial for chromosomal segregation. The regulation of spindle length is well studied, but little is known about spindle width. Previous studies suggested that the spindle can self-organize to maintain a constant aspect ratio between its length and width against physical perturbations. Here we determine the widths of metaphase spindles...

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Just as the size of an organism is carefully controlled, the size of intracellular structures must also be regulated. The mitotic spindle is a supramolecular machine that generates the forces which separate sister chromatids during mitosis. Although spindles show little size variation between cells of the same type, spindle length can vary at least 10-fold between different species. Recent expe...

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ژورنال

عنوان ژورنال: Current Biology

سال: 2008

ISSN: 0960-9822

DOI: 10.1016/j.cub.2008.07.092